Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Application

Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose^® excel medium resistant to chelating and reducing agents

Features and Benefits

• HiTrap^® columns prepacked with Ni Sepharose^® excel medium, resistant to chelating and reducing agents• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity• Increase target protein yield and decrease degradation through reduced and simplified sample handling• HisTrap^™ excel columns allow direct purification of cell-free, unclarified samples.

General description

HisTrap^™ excel columns are prepacked with Ni Sepharose^® excel affinity media for capture and purification of histidine-tagged proteins secreted by immobilized metal ion affinity chromatography (IMAC). The media is resistant to chelating and reducing agents making the medium an excellent choice for the purification of excreted proteins.

Legal Information

HiTrap is a registered trademark of GE Healthcare

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Sepharose is a registered trademark of GE Healthcare

HisTrap is a trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

packaging	pack of 5 × 5 mL
working range	2 - 13
average diameter	90 µm
bed volume	5 mL
column I.D.	16 mm
bed size	16 mm × 25 mm
mfr. no.	GE Healthcare, 17-3712-06
parameter	5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.)
parameter	5 ml/min - 20 ml/min flow rate (2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.)
cleaning	2 - 14 (Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
shelf life	Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
capacity	≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL^® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)
matrix	highly cross-linked 6% agarose

Cas Number

Mixture

This product has met the following criteria to qualify for the following awards:

HISTRAP EXCEL

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